培养基,是指供给微生物、植物或动物(或组织)生长繁殖的,由不同营养物质组合配制而成的营养基质。一般都含有碳水化合物、含氮物质、无机盐(包括微量元素)、维生素和水等几大类物质。培养基既是提供细胞营养和促使细胞增殖的基础物质,也是细胞生长和繁殖的生存环境。
常用规格:35、40、60、70、90、100、110、120、150mm等等。
培养皿是一种用于微生物或细胞培养的实验室器皿,由一个平面圆盘状的底和一个盖组成,一般用玻璃或塑料制成。
简介
培养皿材质基本上分为两类,主要为塑料和玻璃的,玻璃的可以用于植物材料、微生物培养和动物细胞的贴壁培养也可能用到。
塑料的可能是聚乙烯材料的,有一次性的和多次使用的,适合实验室接种、划线、分离细菌的操作,可以用于植物材料的培养。
一个 培养皿(或培养皿 或 细胞培养皿)是一种浅 玻璃 或 塑料 圆柱 盖盘thatbiologists 使用 文化 细胞 [ 1 ] 或小苔藓植物。[ 2 ] 命名为 德国细菌学家Julius Richard Petri 后,[ 3 ] 是谁发明的工作时,作为科赫的助理 。Glass Petri菜可以重复使用bysterilization (例如,在一个 釜 或干加热热风炉在160°C一小时)。对于实验中交叉污染从一个实验,未来会成为一个问题,塑料培养皿中经常被用来作为 耗材。现代的培养皿中往往有眼睑和基地环,使得它们可以堆叠使他们不滑出另一个。多个菜也可以被纳入一个塑料容器,创造所谓的“多井板块”。培养皿有时用于培养细菌。
培养皿常用来为 微生物学研究 琼脂平板 。这道菜是部分充满温暖的液体含有 琼脂 和具体的成分,包括 养分, 血, 盐, 碳水化合物、染料、指标的混合, 氨基酸 和 抗生素。在琼脂冷却和凝固,菜已准备好接受一个微生物拉登样品的过程中被称为 接种 或“镀”。病毒或噬菌体的文化,两步接种是必要的:第一为细菌生长的病毒接种提供主机。
培养板培养倒挂(琼脂上)减少污染的风险从沉降悬浮颗粒和防止冷凝水积聚和扰乱微生物培养。
培养皿也用于真核细胞培养 采用固体琼脂的液体培养基中或。空的培养皿可用于观察植物发芽或小动物的行为,或其他日常实验室的做法,如在烤箱里,携带和存放干燥流体样品。他们的光学透明性和平坦的配置文件借给他们作为一个临时的容器,用于观察样品(尤其是液体的)在低功率显微镜下的共同用途。
(百度翻译)
A Petri dish (or Petri plate or cell culture dish) is a shallow glass or plastic cylindrical lidded dish thatbiologists use to culture cells [1] or small moss plants.[2] It was named after German bacteriologist Julius Richard Petri,[3] who invented it when working as an assistant to Robert Koch. Glass Petri dishes can be reused bysterilization (for example, in an autoclave or by dry heating in a hot air oven at 160°C for one hour). For experiments where cross-contamination from one experiment to the next can become a problem, plastic Petri dishes are often used as disposables.
Modern Petri dishes often have rings on the lids and bases, which allow them to be stacked so that they do not slide off one another. Multiple dishes can also be incorporated into one plastic container to create what is called a "multi-well plate". Petri dishes are sometimes used to culture bacteria.
Petri dishes are often used to make agar plates for microbiology studies. The dish is partially filled with warm liquid containing agar and a mixture of specific ingredients that may include nutrients, blood, salts, carbohydrates,dyes, indicators, amino acids and antibiotics. After the agar cools and solidifies, the dish is ready to receive a microbe-laden sample in a process known as inoculation or "plating." For virus or phage cultures, a two-step inoculation is needed: bacteria are grown first to provide hosts for the viral inoculum.
Petri plates are incubated upside down (agar on top) to lessen the risk of contamination from settling airborne particles and to prevent water condensation from accumulating and disturbing the cultured microbes.
Petri dishes are also used for eukaryotic cell culture in liquid medium or using solid agar. Empty Petri dishes may be used to observe plant germination or small animal behavior, or for other day-to-day laboratory practices such as drying fluids in an oven and carrying and storing samples. Their optical transparency and flat profile lend them to their common use as a temporary receptacle for viewing samples (especially liquid ones) under a low power microscope.
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