V-P试验,又名二乙酰试验。
原理
某些细菌能从葡萄糖→丙酮酸→乙酰甲基甲醇(Acetymethyl
carbinol)→2,3-丁烯二醇(2,3-bytaylene
cylycol),在有碱存在时氧化成二乙酰,后者和胨中的胍基化合物起作用,产生粉红色的化合物。
培养基
含葡萄糖、K2HPO4、蛋白胨各5g,完全溶解于1000ml水中后,分装试管内,间歇灭菌或10磅10分钟灭菌。
方法
1.O’Meara’s法
试剂:40g氢氧化钾溶于100ml蒸馏水中,加入0.3g肌酐即成。
用琼脂培养物将被检细菌接种于葡萄糖蛋白胨水培养后,于35~37℃(哈夫尼亚菌则应在22~25℃)培养48-96小时,。于每毫升培养物中加入0.1ml,猛烈摇振混合。
2.Baritt’s法
试剂:6%α-奈酚酒精溶液为甲液,40%氢氧化钾为乙液。
同上法接种培养细菌,于2ml培养液内加入甲液1ml和乙液0.4毫升,摇振混合。
试验时强阳性者约于5分钟后,可产生粉红色反应,如无反应,应放在36±1℃下培养4h再进行观察。(长时间无反应,可置室温过夜),次日不变者为阴性。
哈哈,我也是复制的,谁记得住啊恶魔猎手- (英雄,城镇中心)
=建造音效=
- At last, we shall have revenge! 终于,我们可以报仇了!
=选定音效=
- The time has come. 是时候了
- We must act! 我们必须行动了!
- My blade thirsts 我的刀刃渴望着……
- Quickly! 快!
- Command Me! 命令我吧!
=行动/执行动作音效=
- [思想控制] You are spellbound!*** 你已经着魔了
- [蜕变] Time to raise hell! 呼唤地狱的时刻到了
- [吸魂] Your soul is mine!*** 你的灵魂属于我
- [鬼怪视界] Hide no longer!*** 你无处可藏
- Anatorettador!** 精灵语
- Though I be damned! 就算我是被诅咒的!
- Duranacal.** 精灵语
- At last. 终于
- Hmmm. 唔
=骂玩家音效=
- I shall fight fire with fire! 我要以火焰来克制火焰。
- Chaos boils in my veins! 混沌的力量在我血液中沸腾
- Demon blood is thicker than... regular blood. 恶魔之血浓于……一般的血
- I like my enemies dead and my blades flaming. 最爱看敌人死在我燃烧的刀刃下
- I love green trees! (注解: 这个很粗暴. 在游戏中其实是倒过来的, 我不想把它拼出来所以我把它倒过来了. )
- You will perish in flames (咳嗽声) Ops, sorry! 你将在火焰中毁灭(咳嗽)哦,对不起!
- Darkness called, ... but I was on the phone, so I missed him. I tried to *69 Darkness, but his machine picked up. I yelled: "Pick up the phone Darkness", but he ignored me. Darkness must have been screening his calls. 黑暗之王一度召唤我,……但我在打电话,所以我错过了。我试着回拨给他,但只听到录音留言。我喊道:“拿起电话呀�诎抵�酢保��撬�焕砦摇:诎抵�跻欢ㄊ瞧帘瘟怂�牡缁啊?br />=攻击音效=
- [.vs 英雄] For Calendor! 为了Kalimdor
- None shall survive! 挡我者死!
- Your blood is mine! 喝你的血!
- Run for your life! 快逃命吧!
- Revenge! 复仇!
丛林守护者- (英雄,城镇中心)
=建造音效=
- I must safegaurd the land! 保护大地是我的职责。
=选定音效=
- Is there danger? 有险情吗?
- Command me. 命令我吧
- Who threatens the wilds? 谁在威胁着大自然?
- The time is now. 就是现在
- Nature is restless. 大自然永不宁静
=行动/执行动作音效=
- [自然之触] Everything I touch dies or comes back to life! 我的触碰带来死亡和再生!
- [扼死藤] This should weed out a few! 这些杂草应该清除
- [荆棘] You mess with the branch, you get the thorns! 你跟树枝战斗,就得面对荆棘。
- [宁静] The calm, before the storm. 暴风雨前的宁静
- By the spirits! 以精灵们的力量!
- For Calendor! 为了Kalimdor!
- Well, there it is. 好,在那边
- So shall is be. 理应如此
- Naturally. 自然而然
=骂玩家音效=
- My father was mounted over someone''s fireplace. 我父亲是画在壁炉上方的座骑
- Feel natural, nature''s way. 感受大自然的自然
- Don''t let the doe hit you on the way out! 那些母鹿要来扁你了
- When I Attack, part 3. 当我攻击时,第3部分
- Free rides for the ladies MM免费骑哦
- Heard up! 收到!
=攻击音效=
- [.vs 英雄] Smite the defilers of the land! 给污染者以致命一击!
- Feel nature''s wrath! 感受自然的愤怒!
- Death to all defilers! 污染者,受死!
- None shall harm the wilds! 没有人可以伤害大自然!
月亮女祭祀- (英雄,城镇中心)
=建造音效=
- Warriors of the night, assemble! 夜之战士们,集结起来!
=选定音效=
- We are poised to strike! 我们随时准备战斗!
- We must act! 我们得行动了!
- I am vigilant! 我时刻警惕着!
- Trust in my command. 相信我的指挥吧!
=行动/执行动作音效=
- [月光] Godess, grant me sight! 女神啊,赐予我慧眼吧!
- [月反射] Let me bounce this off you. 从你身上反弹吧!
- [灼光] Highbeams on! 强光照射!
- [强击] Shoot to kill! 射杀!
- [黎明] Darkness falls across the land. 黑暗降临大地(知道MJ的Thriller吗?Darkness Falls Across The Land ,The Midnight Hour Is Close At Hand,Creatures Crawl In Search Of Blood……''Cause This Is Thriller, Thriller Night……MJ开始与一群僵尸跳舞^o^ )
- Leading the way! 领路!
- Onward! 前进!
- As I thought. 正合我意
- The Godess agrees. 正如女神所愿
=骂玩家音效=
- I command the army of Darkness! 我指挥着黑暗(暗夜)部队!
- I''m caught between the moon and NightElf city. 我往返于月亮与夜精灵城之间
- Come on! We''re burning moonlight. 来吧!我们是燃烧的月之光
- When a Treant falls in the forest, does it make a sound? 一个树人在森林里倒下时,会发出声音吗?
=攻击音效=
- [.vs 英雄] By the Godess! 以女神的力量!
- Strike! 进攻!
- For the Godess! 为了女神!
- Prepare to be Moonstruck!准备接受月神之击!(Moonstruck的字典解释:源于月光能引起精神错乱的信仰,是不是有点讽刺?)
Warden
守望者
Ready - "My prey is near." ---我的猎物就在附近。
Warcry - "Justice has come." ---审判降临。
What1 - "Have you reached a verdict?"---你有判决了吗?
What2 - "Let me investigate." ---让我来仔细调查
What3 - (Elvish)
What4 - (Elvish)
What5 - "I am the iron hand of justice."---我是司法(正义)的铁碗。
Yes1 - "Let the hunt begin."---狩猎开始吧。
Yes2 - "Understood." ---明白。
Yes4 - "I can taste their fear."---我能感觉到他们的恐惧。
Yes5 - "Justice shall be swift." ---执法必须迅速。
YesAttack1 - "They shall not escape!" ---他们决对逃脱不了!
YesAttack2 - "Let justice be served!" ---为司法服务!
YesAttack3 - "I am the law." ---我就是法律。
Pissed1 - "I shall be your executioner." ---我会是你的死刑执行者。
Pissed2 - "Only the guilty need fear me." ---只有犯罪者对我感到恐惧。
Pissed3 - "Justice may be blind, but i'm not." ---司法可能有盲目,但,我没有。
Pissed4 - "Truth, Justice and the Night Elf way." ---执行真理,正义和夜精灵的路线(方法?)
Pissed5 - "You think you're above the law?" ---你认为你能凌驾于法律之上?
Pissed6 - "What we have here is a failure to communicate." ---我们在这所面对的是沟通的失败(既然已经这样,没有沟通的必要了。)
Pissed7 - "I hold you in contempt."---对你,我只有蔑视。(鄙视你!!!:)
Pissed8 - "He's no good to me if he's dead." ---他死了的话,对我毫无价值。(追杀狂。。。)
ORC的:
剑圣- (英雄,城镇中心)
=建造音效=
- I obey the six vengance! (原帖都没翻译...obey是执行的意思,vengance,复仇,报仇,连起来我也不知道.我执行这6个复仇?不通顺...)
=选定音效=
- I am yours! 我属于你!
- Ohh! 噢!
- Yesa, Lord!* 是,主人!
- What task is there?有什么任务?
=行动/执行动作音效=
- I hearo and obey!* 我听从命令!(剑圣说的是日式英语……把r音发成鲁等)
- Hai! 日语,嗨咿!
- Excellent choice! 完美的选择!
- Yes, huh! 是,哈!
=骂玩家音效=
- Snatch the pebble from my hand, grasshopper!
- My a blade can cut through armor, and still cut a tomato!* 我的刀可以切穿盔甲,切到里面的番茄(心脏)
- Twin blade action, for clean, close shave everytime! 看我的双刀流……每次都可以把脸刮的很干净~(剔须刀呀?)
- Wasabi! 日语,芥末 (……剑圣整个一 *** 武士)
=Attack Sounds=
- [.vs 英雄] For the burning Blade! 为了燃烧之刃
- Taste a* my blade! 尝尝我的利刃
- Ailease! *喊声*
- Hooah! *吼声*
先知- (英雄,城镇中心)
=建造音效=
- The future is ours! 未来属于我们!
=选定音效=
- My eyes are open. 我洞悉一切
- Seeing is believing! 眼见为实
- Do you need my counsel? 需要我的忠告吗?
- Destiny awaits. 命运在等待
=行动/执行动作音效=
- It is certain. 确信无疑
- Of course. 当然
- I see. 明白
- Watch out!
=骂玩家音效=
- I see dead people. 我看见死人了(开地图秘籍哦,果然是farseer)
- Touch you tongue to mine! 敢用你的舌头舔我! (狼的呻吟声)
- Concentrate and ask again. 思想集中一点!再问我一遍
- Outlook not so good. 前景不太理想(暗指微软的outlook不太好用)
- Reply hazy. Try again! 回答的摸棱两可,再说一遍!
=攻击音效=
- [.vs 英雄] Spirits of Earth and Storm, strike! 大地与风暴的灵魂啊,攻击吧!
- Strike! 进攻!
- Look out! 注意!
- Attack! 进攻!
牛头人酋长- (英雄,城镇中心)
=建造音效=
- I have an axe to grind! 我有把斧子要磨
=选定音效=
- I stand ready! 我准备好了
- Your command? 你的命令?
- Your order? 你的指示?
- What would you ask of me? 你要我做什么?
=行动/执行动作音效=
- Done! 完成!
- For my ancestors!为了我的祖先们!
- An excellent plan! 好计划!
- Yes Chieftan? 是,酋长?(他自己也是酋长哦)
=骂玩家音效=
- Mmmm. My back is killing me! 唔,我的后背要了我的命。
- I need to take a load off! 我需要减轻负荷。
- Rrrrggg! I think I have a splinter! 呃啊~~~~我想我的骨头碎了!
- These poles are heavy, I should find someone else to tote em!这些柱子好沉啊,我该找别的人来背它(找个真人来作我的图腾)
- I used to have to go to war uphills, both ways!我以前打仗的时候还得上坡,两个方面……
- Your way, right away! 一是你上,二是马上
=攻击音效=
- [.vs 英雄] For the War Chief and the Tribes! 为了酋长和部落!
- For the Tribes! 为了部落!
- Honorguide me!* 荣誉引导着我!
- None shall pass! 把命留下!
Shadow Hunter
暗影猎手
Ready - "Want to see somethin' real scary?" ---想来点真正让人惊慌的玩意儿?
Warcry - "It be the mad time man." ---这真是一个疯狂的时代。
What1 - "Who do I kill first?" ---先来干掉哪个?
What2 - "Where our enemies be hidin'?" ---我们的敌人藏身在哪里?
What3 - "Use my power." ---以我的力量。
What4 - "Direct my blade." ---指引我的利刃。
What5 - "Who be my next victim?" ---接下来谁受死?
Yes1 - "Move faster." ---行动更快些。
Yes2 - "It be a pleasure." ---很乐意。
Yes3 - "Right." ---的确。
Yes5 - "We be jammin'." ---我们陷入了困境。
Yes6 - *laughs*
Yes7 - "Ya man."---年轻人。
YesAttack1 - "My blade be thirsty." ---我的刀刃渴望杀戮。
YesAttack2 - "I pity the fool." ---我真为那些愚蠢的家伙感到可怜。
YesAttack3 - "Killin' be easy." ---将他杀死,简单。
YesAttack4 - "Die!" ---死吧。
Pissed1 - "Keepin it real, up in the feal." ---保持真实,??
Pissed2 - "Your commander is a fool." ---你的指挥官是个傻瓜。
Pissed3 - "Call me for your free reading." ---有免费阅读的话请叫上我。
Pissed4 - "You're the baddest soul in town man." ---你是整个部落最糟糕的灵魂,小子。
Pissed5 - "Humans. They think they know everything." ---人类,他们总以为自己无所不知。
Pissed6 - "You lack discipline." ---你缺乏纪律。
Pissed7 - "Stop whinin'." ---别再抱怨了,年轻人。
Pissed8 - "Look at me. I'm happy." ---看看我。我多开心。
Pissed9 - "Ooh, Baby."---喔,孩子。
UD的:
死亡骑士- (英雄,城镇中心)
=建造音效=
- The pact is sealed! 契约已订!
=选定音效=
- You called? 你召唤我吗?
- My patience has ended! 我的耐心已尽!
- I am the Darkness! 我就是黑暗!
- My vengence is yours! 你就是我的复仇!
=行动/执行动作音效=
- Let battle be joined! 加入战斗!
- As you order! 听从命令!
- Hiyah! 嗨呀!
- At last! 终于!
- For the Lich King!为了巫妖王!
=骂玩家音效=
- Has hell frozen over yet? 地狱还没有冻住吗?
- I am the one horseman of the Apocalypse. 我是天命骑士之一。
- I hate people, but I love gatherings! 我恨人们,但我喜欢收藏品
- I''m a Death Night Rider! Muh ha ha ha 我是一个死亡骑士(死掉的夜骑士)!啊哈哈哈
- Blueca!**
- Don''t touch me... I''m evil! 别碰我……我是邪恶的!
=攻击音效=
- [.vs 英雄] Let terror reign! 让恐惧统治一切吧!
- Feel my rath! 感受我的愤怒!
- Ride or die! 逃跑还是受死!
- By Nazul! 以Ner ''zhul的名义
恐惧之王- (英雄,城镇中心)
=建造音效=
- The night beckons! 夜在召唤!
=选定音效=
- Greetings! 向您问候!
- What, mortal? 什么事,凡人
- What is it now? 现在呢?
- I must hunt soon! 我立即行动!
=行动/执行动作音效=
- That was my plan! 正是我的计划!
- Agreed! 同意!
- Very well. 很好!
- You thought of that? 你也那么想?
=骂玩家音效=
- If I have wings, why am I always walking? 既然我有翅膀,为什么我总是步行?
- (电话铃声) Yes? Arrgghh! For the last time, I''m a Dread Lord, not a Drug Lord! 喂?啊!!我说最后一次,我是一个恐怖魔王,不是毒品王!
- This is not a dress, it''s the standard Dread Lord uniform!这不是一般的服装,这是恐怖魔王标准套装!
- Dress to kill! Blah! 是穿来杀人的!废话!
- (电话铃声) Yes? Darkness, hey, what''s up? The Demon Hunter left you a message? No, I don''t have his number. 喂?黑暗之王吗?嗨,情况如何?恶魔猎手给你留了条短信?哦,不,我没有他的号码。
- And then, after I overthrow this fool... Oh! Hello! I didn''t know you were there. 然后,我揭穿这个笨蛋……啊,喂,我不知道你在那
- Imbisile! 不可思议
=攻击音效=
- [.vs 英雄] Your soul is mine! 你的灵魂属于我!
- I hunger! 我饥渴!
- Deathrageous! 死之怒!
- Die! 死吧!
巫妖- (英雄,城镇中心)
=建造音效=
- The ancient evil survives! 远古邪恶生还了!
=选定音效=
- I am sworn to Nazul! 我效忠于Ner ''zhul !
- Thy bidding? 你的命令?
- Direct me! 指示我吧!
- Yours to command! 由你指挥!
=行动/执行动作音效=
- [清醒] Cllleeaarrr! 了 解!!!
- [冰霜铠甲] Nazul protect them!Ner '
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